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The 2nd response cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, redirected here and water as items (Fig. 9) - UV/Vis/NIR. As attracted Fig - https://visual.ly/users/julieanndesalorenz30606/portfolio. 9, the reaction might also involve electron transfer from H4B to the Fe, IIO2 intermediate
l9 As revealed in Fig. 9, this could be due to the fact that the H4B extreme acts as an electron sink in a later point in the response, so as to allow NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.